Tools
The Dent Lab has generated a few different tools to use for use that are publically available. If you are interested in using any of them they are available through Addgene. If you have additional questions, please contact us
Double UP
Double UP is a Red/Green plasmid used to generate internal controls when combined with a technique such as in utero electroporation. We use this tool frequently, and can offer good support and advice to go with it.
Addgene #125134, 125139
Taylor et al., 2020
MTED
MicroTubule Elimination Domain, this is a small peptide sequence taken from Drosophila Efa6. It is a potent microtubule depolymerizer, and we use it frequently. Attaching it to LifeAct allows for specific targeting to dendritic spines, to inhibit microtubule invasion. We can offer good support and advice to go with it
Addgene #217498, 217499
Holland et al., 2024
TRON
TRON is a computer program designed to quantify neuronal migration on a cell-by-cell basis, and is especially potent when combined with Double UP. We use this tool frequently, but have found that it is especially well suited to the ways in which we image and quantify. It has less broad use than we initially hoped, but we will be happy to teach and offer support to determine if it will work for you
Taylor et al., 2020
Knockdown/Replacement
This is a set of plasmid tools designed to offer shRNA and replacement at roughly endogenous levels, through the use of attenuator sequences. This is not a tool we use currently, and so our support may be more limited.
Addgene #183555-183575
Millette et al., 2022
